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A continuous fluorescence displacement assay for BioA: An enzyme involved in biotin biosynthesis

DJ Wilson, C Shi, BP Duckworth, JM Muretta… - Analytical …, 2011 - Elsevier
Cofactor biosynthetic pathways represent a rich source of potential antibiotic targets. The second
step in biotin biosynthesis is performed by BioA, a pyridoxal 5'-phosphate (PLP) dependent
enzyme. This enzyme has been confirmed as a candidate target in Mycobacterium ...
All 2 versions

Biotin-4-Fluorescein Based Fluorescence Quenching Assay for Determination of Biotin Binding Capacity of Streptavidin Conjugated Quantum Dots

[HTML] from nih.govR Mittal… - Bioconjugate chemistry, 2011 - ACS Publications
The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to
their performance in cell tagging, tracking, and imaging experiments. Commercially available
streptavidin conjugates (SAv QDs) are the most commonly used tool for preparing QD− ...
Related articles - All 4 versions

Biotin functionalized poly (sulfonic acid) s for bioconjugation: In situ binding monitoring by QCM‐D

S Slavin, A De Cuendias, V Ladmiral… - Journal of Polymer …, 2011 - Wiley Online Library
Molecular recognition is of major importance in that it plays a part in many biological
interactions. The avidin–biotin interaction is an excellent model for studying biorecognition due
to the ultra-high binding affinity between them. Avidin is a homotetrameric protein ...
Cited by 1 - Related articles - All 4 versions

Peroxisomes are involved in biotin biosynthesis in Aspergillus and Arabidopsis

Y Tanabe, J Maruyama, S Yamaoka… - Journal of Biological …, 2011 - ASBMB
Aspergillus oryzae. In addition to exhibiting defects in the peroxisomal targeting of either PTS1
or PTS2 proteins, the deletion strains also displayed growth defects on minimal medium containing
oleic acid as the sole carbon source. Unexpectedly, these peroxisomal transport deficient ...
Related articles - All 2 versions

Effects of biotin deficiency on pancreatic islet morphology, insulin sensitivity and glucose homeostasis.

E Larrieta, ML de la Vega-Monroy… - The Journal of …, 2011 - ncbi.nlm.nih.gov
Several studies have revealed that physiological concentrations of biotin are required for the
normal expression of critical carbohydrate metabolism genes and for glucose homeostasis.
However, the different experimental models used in these studies make it difficult to ...
All 2 versions

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