C Luxenburg, HA Pasolli, SE Williams… - Nature Cell Biology, 2011 - nature.com ... (e) Immunoblots were probed with antibodies against β-actin, Wdr1 and α-tubulin. (f) Cell morphology, pERM, cortical actin and/or myosin-IIA are perturbed in keratinocytes expressing Actb or Wdr1 shRNAs, but not scramble control. White lines denote cell borders. ... Cited by 1 - All 4 versions
MA Suni… - Methods in molecular biology (Clifton, NJ), 2011 - Springer ... 12. Washing must be thorough in order to completely remove the perm buffer. Residual perm buffer can negatively impact the staining of PE and PerCP conjugates. 13. Some cell surface epitopes are not maintained under these fixation and permeabilization conditions. ... Cited by 1 - All 2 versions
R Maggio, N Peragine, MS De Propris… - Cancer Immunology, …, 2011 - Springer ... isotypic controls. To assess cytokine production, non-adherent PBMC were analyzed using the Fix and Permcell permeabiliza- tion kit (Caltag Laboratories, Burlingame, CA), according to the manufacturer's instructions. Briefly, non ... Related articles - All 4 versions
ST Buckley, C Medina, M Kasper… - American Journal of …, 2011 - Am Physiological Soc ... Dr. U. Günthert, University of Basel, Institute of Pathology, Basel, Switzerland), diluted 1:10; goat polyclonal anti-RAGE (kind gift from Dr. Michael Neeper, Merck Sharp & Dohme, West Point, PA), diluted 1:1,000; and rabbit polyclonal anti-pERM (Cell Signaling), diluted 1:50. ... Cited by 2 - Related articles - All 4 versions
G Han, S Geng, Y Li, G Chen, R Wang… - …, 2011 - Wiley Online Library ... staining, PE-conjugated anti-mouse CD88 antibody (C5aR; Abcam, Cambridge, MA) were washed and used for flow cytometry analysis; for intracellular (C5L2) staining, the above cells were fixed overnight at 4° with 1 ml fixation buffer (Fix and Permcell permeabiliza- tion kit ... Related articles - All 2 versions
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