W Lin, M Wang, H Jin… - Genetics, 2011 - Genetics Soc America ... 1995; Keeney et al. 1997; Klein et al. 1999). The tetO array was inserted into the URA3 locus on chromosome V, and tetR-GFP at the LEU2 locus on chromosome III, as previously described (Michaelis et al. 1997). A PCR-based strategy (Longtine et al. ... 1997; Michaelis et al. ... Cited by 1 - Related articles - All 3 versions
[HTML] from genetics.orgK Martins-Taylor, U Sharma, T Rozario… - Genetics, 2011 - Genetics Soc America ... To create strain YSH549, hybrid PCR (Horton et al. 1989) was used to link the scc1-73 allele (Michaelis et al. 1997) (amplified from strain KN5832, provided by Kim Nasmyth) to the LEU2 gene from pKMT1, a LEU2 vector designed on the MX-series scaffold (Wach et al. ... Cited by 3 - Related articles - All 3 versions
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