[HTML] from jove.comN Shirole, S Balasubramanian, C Yanofsky… - Journal of Visualized …, 2011 - jove.com ... translational complexes, well established procedures can be used, such as i) crosslinking to specific amino acids 14 and/or ii) alkylation protection assays 1,14,17 . Files. JoVE Article PDF. Protocol. 1. Cell Free ExtractPreparation. ... Related articles - Cached - All 4 versions
G De Jaeger, A Verkest… - US Patent 20,110,162,110, 2011 - freepatentsonline.com ... GE-Healthcare). Protein gel blots were developed by Chemiluminiscent detection (Perkin Elmer, Norwalk, Conn.). Protein ExtractPreparation. Cell material (15 g) was grinded to homogeneity in liquid nitrogen. Crude protein ... Related articles - Cached
AM Rakotondrafara… - Nature Protocols, 2011 - nature.com ... The procedure relies on RNA interference–mediated knockdown of the factor of interest prior to extractpreparation, and it overcomes problems with the availability and specificity of antibodies, as well as with the co‐depletion of proteins associated with the factor under study. ... All 3 versions
X Bai, K Chmura, AR Ovrutsky, RP Bowler… - Tuberculosis, 2011 - Elsevier ... CGC TGT TGA AGT CAG AGGA-3′ (antisense). All PCR findings were normalized to GAPDH expression. 2.6. Nuclear extractpreparation and electrophoretic mobility shift assay. THP-1 cells were infected with MTB H37Rv ... Cited by 2 - Related articles - All 3 versions
[HTML] from nih.govKA Amin, MS Hassan, EST Awad… - International Journal of …, 2011 - ncbi.nlm.nih.gov ... after MCT administration. MCT with or without concurrent CeO 2 effects was evaluated. Hepatic cytosolic and mitochondrial extractpreparation. The liver was rinsed with cold PBS to remove excess blood. Small portions of the ... Cited by 1 - Related articles - All 7 versions
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